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Through scRNA-seq analysis two TAN subsets, named Neutrophil 1 and Neutrophil 2, expressing different gene signatures have been detected in the metastatic BM microenvironment ( CXCR1 and CXCR2 they are attracted by chemokines produced by tumor cells and infiltrate the TME (107)
Eventually, I got to join them but I could not cast as far or as straight (my uncle Henry was a stickler for a straight cast and would not hesitate to cut your line as soon as it drifted over his, a lesson learnt quickly)
You can use a steady retrieval and experiment with speeds or try jigging or twitching it for more interest
Then well go into a few variations that may give you some ideas about how to improve your own setup
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