CMBS - Calcium-free Modified Barth Solution (500 ml) - 10x concentrate Na2HPO4 = 10 mM

Na2HPO4 = 10 mM

NaCl = 137 mM

Ional composition of CC-BSCS Solution

For 1000 ml PBS Solution:

NaHCO3 = 25 mM

CMBS - Calcium-free Modified Barth Solution (500 ml) - 10x concentrate Na2HPO4 = 10 mMCalcium free Modified Barth's Solution (CMBS) is used for defolliculation of Xenopus laevis oocytes. Product information 10x concentrate CMBS solution autoclaved Instructions for use: 10x concentrate: For 1000 ml CMBS Solution: 1. Pour 100 ml CMBS (10x) into a flask 2. Add deionized water to a final volume of 1000 ml Adjust to pH 7. 4 Use immediately once opened. Store at 4 8 C Ional composition of CMBS Solution NaCl = 88 mM KCl = 1 mM MgSO4 = 0,8 mM