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The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-23
Then a biotinylated detection antibody specific for Human ALB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL1α2
You can calculate the concentration of Porcine CTR in the samples by comparing the OD of the samples to the standard curve
The concentration of Mouse BNP in the samples is then determined by comparing the OD of the samples to the standard curve
MBP-Tag Mouse mAb Packaging:100 uL The micro ELISA plate providedMaltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP fusion protein, preventing aggregation of
