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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IL1a
And then biotinylated Human SLC2A10 Antibody is added and binds to SLC2A10 in the sample
The OD value is proportional to the concentration of Rat CASP3
Human CD147 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CD147
Then a biotinylated detection antibody specific for Mouse FE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human SMAD4 (Mothers Against Decapentaplegic Homolog 4) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (SMAD4) Mothers Against Decapentaplegic Homolog 4 ELISA Kit measures Mothers Against Decapentaplegic Homolog 4 in samples. The plate has been pre coated with Human SMAD4 antibody. SMAD4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SMAD4 Antibody is added and binds to SMAD4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SMAD4 antibody. After incubation
