Human ANXA1 (Annexin A1) ELISA Kit Packaging:96 wells only those wells that contain

only those wells that contain Human FLNC

The concentration of Mouse IL34 in the samples is then determined by comparing the OD of the samples to the standard curve

The concentration of Sheep IFNb in the samples is then determined by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Human sC5b-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LGALS3BP

Human ANXA1 (Annexin A1) ELISA Kit Packaging:96 wells only those wells that containThe Human (ANXA1) Annexin A1 ELISA Kit measures Annexin A1 in samples. The plate has been pre coated with Human ANXA1 antibody. ANXA1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ANXA1 Antibody is added and binds to ANXA1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ANXA1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.