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Universal SP in the sample or standard competes with a fixed amount of Universal SP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal SP
The OD value is proportional to the concentration of Mouse POSTN
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E2
The OD value isproportional to the concentration of Human IL-6
Then a biotinylated detection antibody specific for Universal TGF-Beta 1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human TGF-Beta3 (Transforming Growth Factor Beta 3) ELISA Kit Packaging:48 wells Universal SP in the sampleThe Human TGF Beta3 (Transforming Growth Factor Beta 3) ELISA kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human TGF Beta3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGF Beta3 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively
