Rat ERFE (Erythroferrone) ELISA Kit Packaging:96 wells and the color intensity is

and the color intensity is directly proportional to the amount of Rat PVRL1 present

only those wells that contain Human HPR

Substrate solution is then added and color develops in proportion to the amount of Rat FLOT1

And then biotinylated Human CSTA Antibody is added and binds to CSTA in the sample

CEACAM6 present in the sample is added and binds to antibodies coated on the wells

Rat ERFE (Erythroferrone) ELISA Kit Packaging:96 wells and the color intensity isThe Rat (ERFE) Erythroferrone ELISA Kit measures Erythroferrone in Rat samples. The plate has been pre coated with Rat ERFE antibody. ERFE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ERFE Antibody is added and binds to ERFE in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ERFE antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.