Human HIG2 (Hypoxia-inducible gene 2 protein) ELISA Kit Packaging:96 wells C6orf105 present in the sample

C6orf105 present in the sample is added and binds to antibodies coated on the wells

The plate has been pre-coated with Rat OXTR antibody

Then a biotinylated detection antibody specific for Rat IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Then a biotinylated detection antibody specific for Human EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The concentration of Human TGFb3 in the samples is then determined by comparing the OD of the samples to the standard curve

Human HIG2 (Hypoxia-inducible gene 2 protein) ELISA Kit Packaging:96 wells C6orf105 present in the sampleThe Human HIG2 (Hypoxia inducible gene 2 protein) ELISA Kit measures HIG2 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HIG2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated