Human Interleukin-13 (IL-13) ELISA Kit NTN The enzyme-substrate reaction is terminated

The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow

Mouse IgG3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse IgG3

Then a biotinylated detection antibody specific for Rat KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

only those wells that contain Rat IL34

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human XCL2

Human Interleukin-13 (IL-13) ELISA Kit NTN The enzyme-substrate reaction is terminatedThis ELISA kit is designed to detect Human Interleukin 13 (Human IL 13). The assay plate has been pre coated with mouse anti Human IL 13 monoclonal antibody. When the sample containing IL 13 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti Human IL 13 Antibody is added to the wells and binds to IL 13 in the sample. After washing the wells, substrate solutions are added, and the