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Then Streptavidin-HRP is added and binds to the Biotinylated RELN antibody
Then a biotinylated detection antibody specific for Universal A-CoA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Bovine MB antibody
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions
Human ACO1 (Aconitase 1) ELISA Kit G0S19- Then Streptavidin-HRP is added andThe Human ACO1 (Aconitase 1) ELISA Kit measures ACO1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ACO1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.