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Substrate solution is then added and color develops in proportion to the amount of Human HP
The plate has been pre-coated with Rat SERPINE1 antibody
CA7 present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Human CSTB Antibody is added and binds to CSTB in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GDF6
Human STX6 (Syntaxin 6) ELISA Kit SF Substrate solution is then addedThe Human (STX6) Syntaxin 6 ELISA Kit measures Syntaxin 6 in samples. The plate has been pre coated with Human STX6 antibody. STX6 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human STX6 Antibody is added and binds to STX6 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated STX6 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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