DEFa3 (Defensin Alpha 3, Neutrophil Specific) ELISA Kit (Small Sample) nuclear receptor subfamily 1 a horseradish peroxidase conjugated mouse

a horseradish peroxidase conjugated mouse anti-Human RELT Antibody is added to the wells and binds to RELT in the sample

The concentration of Mouse TNF-alpha in the samples is then determined by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Human suPAR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Then a biotinylated detection antibody specific for Mouse MAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

and the color intensity is directly proportional to the amount of Human interferon-alpha present

DEFa3 (Defensin Alpha 3, Neutrophil Specific) ELISA Kit (Small Sample) nuclear receptor subfamily 1 a horseradish peroxidase conjugated mouseThe DEFa3 (Defensin Alpha 3, Neutrophil Specific) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human DEFa3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human DEFa3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB