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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GS
And then biotinylated Human CS Antibody is added and binds to CS in the sample
And then biotinylated Canine IL18 Antibody is added and binds to IL18 in the sample
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LTF
Substrate solution is then added and color develops in proportion to the amount of Rat CASP2
Human PAP (Prostatic Acid Phosphatase) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (PAP) Prostatic Acid Phosphatase ELISA Kit measures Prostatic Acid Phosphatase in samples. The plate has been pre coated with Human PAP antibody. PAP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PAP Antibody is added and binds to PAP in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PAP antibody. After incubation unbound Streptavidin HRP is washed away
