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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SIRT1
Then a biotinylated detection antibody specific for Mouse MUC5AC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Mouse GRIN2A antibody
You can calculate the concentration of Human IL-12 in the samples by comparing the OD of the samples to the standard curve
Only those wells that contain Rat sCD14
Porcine CKM (Creatine Kinase M-type) ELISA Kit Packaging:96 wells Standards or samples are addedThe Porcine (CKM) Creatine Kinase M type ELISA Kit measures Creatine Kinase M Type in Porcine samples. The plate has been pre coated with Porcine CKM antibody. CKM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine CKM Antibody is added and binds to CKM in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CKM antibody. After incubation unbound Streptavidin HRP is washed away
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