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The plate has been pre-coated with Rat GUCA2A antibody
it binds with C-P bound to precoated antibody
Substrate solution is then added and color develops in proportion to the amount of Human ASIP
Unbound reagent is then washed out
Then a biotinylated detection antibody specific for Rat GROGamma/CXCL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Canine THBS1/TSP-1 (Thrombospondin 1) QuickTest ELISA Kit Packaging:96 wells The plate has been pre-coatedThe Canine THBS1 TSP 1 (Thrombospondin 1) QuickTest ELISA Kit measures THBS1 TSP 1 in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples
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