Mal-Acetyl-Dap(m-PEG4)-Val-Cit-PAB(m-PEG4)-PNP Size:25mg 732 SMILES OC(=O)CN1CCN(CC(O)=O)CCN(CC(=O)NCCOCCOCCNC(=O)CCN2C(=O)C=CC2=O)CCN(CC(O)=O)CC1 Purity (HPLC)

732 SMILES OC(=O)CN1CCN(CC(O)=O)CCN(CC(=O)NCCOCCOCCNC(=O)CCN2C(=O)C=CC2=O)CCN(CC(O)=O)CC1 Purity (HPLC) >= 95% Shipping Conditions Room temperature Storage Conditions Refrigerated Shelf Life 12 months after the date of delivery Regulatory Statement For Research Use Only

235 SMILES BrCCOCCOC1=C2C(=O)N(C3CCC(=O)NC3=O)C(=O)C2=CC=C1 Purity (HPLC) >= 95% Shipping Conditions Room temperature Storage Conditions Refrigerated Shelf Life 12 months after the date of delivery Regulatory Statement For Research Use Only

73 SMILES: CCN(CC)C1=CC=C2C(OC3=CC(C=CC3=C2C2=CC=C(C=C2S([O-])(=O)=O)S(=O)(=O)NCC#C)=[N+](CC)CC)=C1 Purity (HPLC): ≥ 95% Shipping Conditions: room temperature Storage Conditions: Refrigerated Regulatory Statement: For Research Use Only

Compared to earlier iterations

Unlike traditional chemical reactions utilizing succinimidyl esters or maleimides that target amines and sulfhydryls – functional groups that are not unique – click chemistry-labeled molecules can be applied to complex biological samples and be detected with unprecedented sensitivity due to extremely low background

Mal-Acetyl-Dap(m-PEG4)-Val-Cit-PAB(m-PEG4)-PNP Size:25mg 732 SMILES OC(=O)CN1CCN(CC(O)=O)CCN(CC(=O)NCCOCCOCCNC(=O)CCN2C(=O)C=CC2=O)CCN(CC(O)=O)CC1 Purity (HPLC)SOLU KNEX cleavable linker features a Val Cit dipeptide. In this product, a Mal Acetyl Dap(m PEG4) moiety replaces the conventional maleimidocaproyl (MC) unit to enhance aqueous solubility. The Mal Acetyl group may also improve retro Michael addition stability during antibody conjugation. Additionally, m PEG4 is attached to the commonly used self immolative para aminobenzyl (PAB) moiety to further increase aqueous solubility. The PNP (para