Rat MGP (Matrix Gla Protein) ELISA Kit Packaging:96 wells then added a HRP-conjugated antibody

then added a HRP-conjugated antibody specific to Human FE

Substrate solution is then added and color develops in proportion to the amount of Human AOC3

Then Streptavidin-HRP is added and binds to the Biotinylated BMP5 antibody

Substrate solution is then added and color develops in proportion to the amount of Rat NPW

Unbound of anti-Estradiol Biotin Reagent and E2 peroxidase conjugate is then removed and the wells are washed

Rat MGP (Matrix Gla Protein) ELISA Kit Packaging:96 wells then added a HRP-conjugated antibodyThe Rat (MGP) Matrix Gla Protein ELISA Kit measures Matrix Gla Protein in Rat samples. The plate has been pre coated with Rat MGP antibody. MGP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MGP Antibody is added and binds to MGP in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MGP antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.