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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CEA are added to the micro ELISA plate wells
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 39 nm
The standards
The plate has been pre-coated with Human HOXA10 antibody
and the color intensity is directly proportional to the amount of Human Prolactin Receptor present
Human UNC5B (Netrin Receptor Unc5B) ELISA Kit Packaging:96 wells Samples (or Standards) and HorseradishThe Human (UNC5B) Netrin Receptor Unc5B ELISA Kit measures Netrin Receptor UNC5B in samples. The plate has been pre coated with Human UNC5B antibody. UNC5B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human UNC5B Antibody is added and binds to UNC5B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated UNC5B antibody. After incubation unbound Streptavidin HRP is washed away
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