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Then a biotinylated detection antibody specific for Human IL-1R2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Human EGFR2
a horseradish peroxidase conjugated mouse anti-Rat IL1R2 Antibody is added to the wells and binds to IL1R2 in the sample
The concentration of Cattle EPO in the samples is then determined by comparing the OD of the samples to the standard curve
The Mouse Flt3L (FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit is a Sandwich enzyme immunoassay
Canine CAV-1 (Caveolin-1) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Canine (CAV 1) Caveolin 1 ELISA Kit measures Caveolin 1 in Canine samples. The plate has been pre coated with Canine CAV1 antibody. CAV1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine CAV1 Antibody is added and binds to CAV1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CAV1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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